NADPH
Tetrasodium salt |
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| Empirical Formula (Hill Notation): |
C21H26N7Na4O17P3 · xH2O |
| Synonym(s): |
β-NADPH, Coenzyme II reduced tetrasodium salt, 2′-NADPH hydrate, β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, Triphosphopyridine nucleotide reduced tetrasodium salt, NADPH Na4, TPNH2 Na4, Dihydronicotinamide adenine dinucleotide phosphate tetrasodium salt |
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| CAS Number: |
2646-71-1 |
| Molecular Weight: |
833.35 (anhydrous basis) |
| Assay |
≥97% (dry weight) |
| Form |
powder |
| Packaging |
pkg of 1 g (10621706001) |
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pkg of 100 mg (10107824001) |
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pkg of 500 mg (10621692001) |
| λmax |
260 and 340 nm |
| Shipped in |
ice bag |
| Storage temp. |
2-8°C |
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| DESCRIPTION |
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| General description |
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| Amorphous powder bottled under nitrogen. |
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| Absorbance coefficients: 15.0 x 103 l x mol-1 x cm-1 at 260 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm |
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| Absorbance maxima: 260 nm and 340 nm |
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| Molecular weight: NADPH-Na4: Mr = 833.4, NADPH: Mr = 745.4 |
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| pH value in water: 8.0 ± 1.0 (10 mg/ml) |
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| Application |
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| NADPH-RO has been used for the determination of thioredoxin reductase activity. |
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| Biochem/physiol Actions |
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| NADPH is an electron donor and a cofactor for many redox enzymes including nitric oxide synthetase. |
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| Quality |
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| Purity: =97% ß-NADPH-Na4 based on dry weight (enzymatically and absorbance at 340 nm), =80% (absorbance at 260 nm) |
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| Organic solvents: =3% (GC) |
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| Preparation Note |
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| Storage conditions (working solution): 2 to 8 °C |
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| 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADPH after 4 weeks at 2 to 8 °C. |
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| Analysis Note |
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| Absorbance coefficients: 15.0 x 103 l x mol-1 x cm-1 at 260 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm |
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| Other Notes |
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| Packaged based on solid weight. |
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| For life science research only. Not for use in diagnostic procedures. |
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